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Journal: Frontiers in Physiology

Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

doi: 10.3389/fphys.2026.1781613

Figure Lengend Snippet: Primer sequences for the genes observed in qRT-PCR.

Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

Techniques:

Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

Journal: Frontiers in Physiology

Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

doi: 10.3389/fphys.2026.1781613

Figure Lengend Snippet: Effects of HH on the BBB integrity in mouse hippocampus. (A) Representative images of EB extravasation in the hippocampus. (B) Quantitative analysis of EB leakage. (C) Representative western blots of ZO-1 and occludin. Quantitative analysis of the ZO-1/β-actin (D) and occludin/β-actin (E) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For EB, n = 6 per group; for Western blot analysis, n = 3 per group, ** p < 0.01 vs. Con group.

Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

Techniques: Western Blot

Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

Journal: Frontiers in Physiology

Article Title: Transcriptomic analysis and experimental verification reveal the involvement of PI3K/AKT signaling pathway in high-altitude cognitive dysfunction

doi: 10.3389/fphys.2026.1781613

Figure Lengend Snippet: Effects of HH on the expression of genes and proteins related to PI3K/AKT signaling pathway in mouse hippocampus. (A) The relative mRNA expression levels of Kdr , Spp1 , Vwf , and Vegfa screened via qRT-PCR. (B) Representative western blots of p-PI3K, PI3K, p-AKT, and AKT. Quantitative analysis of the p-PI3K/PI3K (C) and p-AKT/AKT (D) ratios. (E) Representative western blots of Nrf2, HO-1, p-NF-κB, and NF-κB. Quantitative analysis of the Nrf2/β-actin (F) , HO-1/β-actin (G) , and p-NF-κB/NF-κB (H) ratios. Data are presented as mean ± SD. Statistical comparisons were performed by Student’s t test. For qRT-PCR, n = 6 per group; for Western blot analysis, n = 3 per group, * p < 0.05, ** p < 0.01 vs. Con group.

Article Snippet: Primary antibody against β -actin were purchased from Zhongshan Golden Bridge Biological Technology Co., Ltd. (Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot